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Ultra High Power LED Light Source
for Optogenetics and Fluorescence Microscopy
UHP-Mic-LED-460
 
 

Introduction

   The Ultra High Power LED provides almost X10 increase of power in comparison with similar High power LED devices. The Ultra High Power LED ( > 36Watt) is an effective replacement of lasers and lamps in many power demanding applications, such as optogenetics, fluorescence microscopy, machine vision, chemical reaction activation and numerous others

    In Optogenetics the LED can be used for both in-situ and in-vivo applications. In in-situ experiments the LED can be connected to epifluorescence port of microscope, in in-vivo experiments the LED can be used with fiber coupler adaptor to enable opsin activation through fiber optic ferule in freely moving animals.

    In fluorescence microscopy such markers as GFP, Quantum dots and other blue wavelength excitation markers can be used with this ultra-high power LED light source. The 460 nm LED will supply the needs of such power hungry methods as Photostimulation of Channelrhodopsin (ChR1, ChR2), FISH and FRAP.

  This new member of the modular Optiblocks family provides >2.2W of collimated LED power on its output and can be used with a full range of other optical OptiBlocks such as Fiber Coupler, Liquid Light Guide coupler, Beam Combiner, Filter Wheel, Beam Switcher and others.

  Please see full list of Optional Accessories

  See other Ultra-High-Power-LED wavelengths

Ultra-High-Power-LED-Microscope
 
UHP LED Fiber Coupled
 
 
 

Key features

  • Compatible with Prizmatix Optogenetics Toolbox and modular Microscope-LED Light Source products families
  • Single chip Ultra High Power LED
  • Collimated, Fiber Coupled or Light Guide options of light delivery
  • Optically isolated TTL input for external triggering
  • Analog input for light power control (optional)
  • Excellent for fluorescence excitation
  • LED spectrum can be narrowed by optional band pass filter installed inside the head
  • Stable precisely adjustable power
  • Long life (no lamp or laser tube replacement required)
  • Speckle free
  • Rapid warm up time
 
 

Applications

  • Optogenetics: Photostimulation of Channelrhodopsin ChR1, ChR2
  • Excitation of GFP, Quantum Dots, di-8-ANEPPS, Auramine
  • Whole body imaging
  • Photo activation (PA)
  • Fluorescence recovery after photo bleach (FRAP)
  • Fluorescence in-situ hybridization (FISH)
  • Bio analysis
  • Machine vision
 
 

Optical Specifications

 
 
Wavelength ±5 460
Wavelength range nm ±5
Spectrum FWHM nm 27
Collimated optical power output Watt >2.2
                         
 
 

Benchtop LED Controller Specifications


 
   Features:
  • Constant current or chopping modes
  • Precise LED current setting by 10 turn dial
  • TTL external trigger input
  • Compact and robust enclosure
  
Digital modulation input TTL
Connector fo TTL input BNC
Digital modulation frequency Hz DC-30000
Rise /Fall time (10%-90%) µs 6
Input Voltage V 12
Max Input current A 5.5
Power Adaptor Input 100-240 VAC, 50-60Hz,1.5A
 
 

General Specifications

 
 
  
Operation temperature range 0C 10-35
Storage temperature range 0C -10-55
Operative relative humidity (Non condensing) % <90
Head dimensions See drawing below
Head weight g 500
Controller dimensions (LxWxH) 220mm x 165mm x 51.5mm
Controller weight g 700
Power adaptor dimensions (LxWxH) 146mm x 76mm x 44mm
Power adaptor weight g 600
Fan noise dBA 38
 
 

Performance

 
 
UHP-Mic-LED-460 spectrum
 
 

Optional Accessories

 

  Fibre Couppled Adapator:
The Microsocpe-LED can be easily changed from direct microscope coupling to fiber coupled LED configuration by means of Fiver Coupled Adaptor.This adaptor can be easily assembled by a user on the Mic-LED output. Please see video clip and contact us for more details.

 
Liquid Light Guide Adaptor:
The Microscope-LED can be easily changed from direct microscope coupling to Liquid Light Guide coupled LED configuration by means of LIGA Adaptor.This adaptor can be easily assembled by a user on the Mic-LED output. Please see video clip and contact us for more details.
 
Fiber patch cords:
Various fiber optic patch cords are available fpr use with the 3-LED or 5-LED products. Most popular patch cords are 1000 / 1500 / 2000 micron core diameter Polymer Optical Fibers (POF) terminated by optical SMA connectors on both sides. Prizmatix can provide Stainless Steel tube at one end of the patch instead of SMA connector. This configuration is more convenient in some applications See more info...
 
Collimator::
The output from optical fiber is divergent according to fiber NA. In order to reduce the divergent angle a collimator module can be used. Prizmatix collimator was especially designed to fit thick core high NA Polymer Optical Fivers. See more info...
 
Fiber Bundles::
To combine output of multiple LEDs a Y-shaped fiber bundle with two or more input branches can be used. Prizmatix can help to configure and build custom fiber bundles for specific applications. See more info...
 
Special Bundles::
For spectroscopy application special fiber optic bundles are very important. Special care must be addressed to eliminate possible cross link between the excitation snd the collection fibers.
See more info...
 
 

Mechanical drawings


 

Application Examples:



Optogenetics  LED Light Source:

   Optogenetics illumination sources include lasers and LEDs, and photoactivation can be done under a microscope or via a fiber for in-vitro or in-vivo applications. Using a Prizmatix LED illumination system for optogenetics studies provides many advantages compared to laser-based systems.
Read more... 

GFP Excitation Light Source:

  The Green Fluorescent Protein GFP is one of many recently developed fluorescent proteins used to observe cells over time expressing one or more proteins tagged with fluorescent marker. The Ultra High Power LED is excellent light source to be used with GFP fluorescent markers in fluorescence microscopy. The excitation wavelength of GFP is in great match with the emission wavelength of UHP-Mic-LED-460. The standard GFP filter cube can be used to observe GFP fluorescence

© Prizmatix, Israel