UHP-Mic-LED-475 Ultra High Power LED Light Source for Fluorescence Microscopy


  The Ultra High Power LED 475 nm provides excellent replacement of conventional arc lamp sources in fluorescence microscopy applications like FITC, GFP and Rhodamine stain. Many power demanding applications such as chemical reaction activation, machine vision, and numerous others may benefit of this new LED light source.

  This new member of the modular Optiblocks family provides >2.7W of collimated LED power on its output and can be used with a full range of other optical OptiBlocks such as Fiber Coupler, Liquid Light Guide coupler, Beam Combiner, Filter Wheel, Beam Switcher and others.

  Please see full list of Optional Accessories

  The LED driver supports CW or pulsed operation.

  See other Ultra-High-Power-LED wavelengths


Key features

  • Compatible with Prizmatix modular Microscope-LED Light Source products family
  • TTL external triggering
  • Excellent for fluorescence excitation
  • LED spectrum can be narrowed by optional band pass filter
    istalled inside the head
  • Stable precisely adjustable power
  • Long life (no lamp or laser tube replacement required)
  • Speckle free
  • Rapid warm up time


  • Fluorescence Microscopy with fluorophores CFP, Rhodamine, FITC, Oregon Green 488, Alexa Fluor 488
  • Whole body imaging of small animals in-vivo
  • Photo activation (PA)
  • Fluorescence recovery after photo bleach (FRAP)
  • Fluorescence in-situ hybridization (FISH)
  • Bio analysis
  • Machine vision

Optical Specifications

Wavelength nm 475
Wavelength range nm ±5
Spectrum half width nm 28
Collimated optical power output W >2.7

Benchtop LED Controller Specifications

  • Constant current or chopping modes
  • Precise LED current setting by 10 turn dial
  • TTL external trigger input
  • Compact and robust enclosure
Digital modulation input TTL
Connector fo TTL input BNC
Digital modulation frequency Hz DC-30000
Rise /Fall time (10%-90%) μs 6
Input Voltage V 12
Max Input current A 5.5
Power Adaptor Input 100-240 VAC, 50-60Hz,1.5A

General Specifications

Operation temperature range 0C 10-35
Storage temperature range 0C -10-55
Operative relative humidity (Non condensing) % <90
Head dimensions See drawing below
Head weight g 500
Controller dimensions (LxWxH) 220mm x 165mm x 51.5mm
Controller weight g 700
Power adaptor dimensions (LxWxH) 146mm x 76mm x 44mm
Power adaptor weight g 600
Fan noise dBA 38


UHP-Microscope-LED-475 Spectrum

Mechanical drawings


Optional Accessories


Filter Wheel:
  The UHP-Mic-LED can be equipped with a 6 positions filter wheel at the beam output. This accessory is especially useful for UHP-Mic-LED-White light source. For more details please see video clip
  Fibre Coupled Adapator:
  The UHP-Mic-LED can be easily changed from direct microscope coupling to fiber coupled LED configuration by means of Fiver Coupled Adaptor (SMA,CF or ST connector).This adaptor can be easily assembled by a user on the Mic-LED output. For more details please see video clip

Liquid Light Guide Adaptor:
  The Microscope-LED can be easily changed from direct microscope coupling to Liquid Light Guide coupled LED configuration by means of LIGA Adaptor.This adaptor can be easily assembled by a user on the Mic-LED output. For more details please see video clip
  The output from optical fiber is divergent according to fiber NA. In order to reduce the divergent angle a collimator module can be used. Prizmatix collimator was especially designed to fit thick core high NA Polymer Optical Fivers. For more details please see http://www.prizmatix-led.com/optics/collimator.htm
Fiber Bundles::
  To combine output of multiple LEDs a Y-shaped fiber bundle with two or more input branches can be used. Prizmatix can help to configure and build custom fiber bundles for specific applications.For more details please see http://www.prizmatix-led.com/dev/Custom-Fiber-Optic-Assemblies.htm

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