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UHP-Mic-LED-595 - Ultra High Power LED Light Source
for Fluorescence Microscopy
 
 

Introduction

    The ULtra High Power (22Watt) LED 595 nm provides excellent replacement of conventional lamp sources in fluorescence microscopy. Many applications such as activation of light-driven ion pump like Halorhodopsin (NpHR) in Optogenetics may benefit of this new LED light source.

  This new member of the modular Optiblocks family provides >650mW of collimated LED power on its output and can be used with a full range of other optical OptiBlocks such as Fiber Coupler, Liquid Light Guide coupler, Beam Combiner, Filter Wheel, Beam Switcher and others.
Please see full list of Optional Accessories

  The LED driner supports CW or fast TTL triggered operation

    In Optogenetics the 595 nm LED can be used for both in-situ and in-vivo activation of Halorhodopsin (NpHR). In in-situ experiments the LED can be connected to epifluorescence port of microscope, in in-vivo experiments the LED can be used with fiber coupler adaptor to enable opsin activation through fiber optic ferule.

    In fluorescence microscopy such fluorophores as Alexa Fluor 594, Texas Red, DyLight 594 and other yellow wavelength excitation markers can be used with this ultra-high power LED light source.

See other Ultra-High-Power-LED wavelengths

UHP-Mic-LED-405nm UHP LED Fiber Coupled
 
 
 

Key features


  • Compatible with Prizmatix Optogenetics Toolbox and
    modular Microscope-LED Light Source products families
  • TTL external triggering
  • Excellent for fluorescence excitation
  • Stable precisely adjustable power
  • Long life (no lamp or laser tube replacement required)
  • Speckle free
  • Rapid warm up time
 
 

Applications

  • Optogenetics - LED activation of Halorhodopsin (NhPR)
  • Fluorescence Microscopy with fluorophores Alexa Fluor 594, Texas Red, DyLight 594
  • Whole body imaging of small animals in-vivo
  • Photo activation (PA)
  • Bio analysis
  • Machine vision
 
 

Optical Specifications

 
 
  
Wavelength nm 595
Wavelength range nm ±5
Spectrum half width nm 16
Collimated optical power output mW >650
                         
 
 

Benchtop LED Controller Specifications


 
   Features:
  • Constant current or chopping modes
  • Precise LED current setting by 10 turn dial
  • TTL external trigger input
  • Compact and robust enclosure
  
Digital modulation input Optically isolated TTL
Connector fo TTL input BNC
Digital modulation frequency Hz DC-20000
Rise /Fall time (10%-90%) µs 6
ON /OFF extinction ms >106:1
Input Voltage V 12
Max Input current A 5.5
Power Adaptor Input 100-240 VAC, 50-60Hz,1.5A
 
 

General Specifications

 
 
  
Operation temperature range 0C 10-35
Storage temperature range 0C -10-55
Operative relative humidity (Non condensing) % <90
Head dimensions See drawing below
Head weight g 500
Controller dimensions (LxWxH) 220mm x 165mm x 51.5mm
Controller weight g 700
Power adaptor dimensions (LxWxH) 146mm x 76mm x 44mm
Power adaptor weight g 600
Fan noise dBA 38
 
 

Performance

 
 
UHP-Mic-LED-595nm
UHP-Microscope-LED-595 spectrum
 
 

Mechanical drawings


 
 
 

Optional Accessories

 

 
Filter Wheel:
The UHP-Mic-LED can be equipped with a 6 positions filter wheel at the beam output. This accessory is especially useful for UHP-Mic-LED-White light source. For more details please see video clip
 
  Fibre Coupled Adapator:
The UHP-Mic-LED can be easily changed from direct microscope coupling to fiber coupled LED configuration by means of Fiver Coupled Adaptor (SMA,CF or ST connector).This adaptor can be easily assembled by a user on the Mic-LED output. For more details please see video clip

 
Liquid Light Guide Adaptor:
The Microscope-LED can be easily changed from direct microscope coupling to Liquid Light Guide coupled LED configuration by means of LIGA Adaptor.This adaptor can be easily assembled by a user on the Mic-LED output. For more details please see video clip
 
Collimator:
The output from optical fiber is divergent according to fiber NA. In order to reduce the divergent angle a collimator module can be used. Prizmatix collimator was especially designed to fit thick core high NA Polymer Optical Fivers. For more details please see http:www.prizmatix-led.com/optics/collimator.htm
 
Fiber Bundles::
To combine output of multiple LEDs a Y-shaped fiber bundle with two or more input branches can be used. Prizmatix can help to configure and build custom fiber bundles for specific applications. For more details please see http:www.prizmatix-led.com/dev/Custom-Fiber-Optic-Assemblies.htm

 

 

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